Former Lab Members

Former Scientists

Dima Klenchin

Scientist and Lab Manager 2013 - 2018  

Current Status (as of  October 2018)- Scientist, PPD

klenchin@wisc.edu | (608) 262-8580 |

Former Postdocs

Marcel Goldschen-Ohm

Postdoc   2009-2017

Current Position: Assistant Professor, Department of Neuroscience, University of Texas at Austin
Visit Marcel’s lab page here.

Lab Publications:

  • Marcel P. Goldschen-Ohm, Baron Chanda, SnapShot: Channel Gating Mechanisms, Cell, Volume 170, Issue 3, 2017, Pages 594-594.e1, ISSN 0092-8674, http://dx.doi.org/10.1016/j.cell.2017.07.019.
  • Zhao, Y., Goldschen-Ohm, M.P., Morais-Cabral, J.H., Chanda, B., and Robertson, G.A. (2017) The intrinsically liganded cyclic nucleotide-binding homology domain promotes KCNH channel activation. Journal of General Physiology DOI:10.1085/jgp.201611701.
  • Goldschen-Ohm, M.P., White, D., Klenchin, V., Chanda. B., and Goldsmith, R. (2017) Observing single molecule dynamics at millimolar concentrations. Angew. Chem. Int. Ed. DOI:10.1002/anie.201612050.
  • Goldschen-Ohm MP, Klenchin VA, White DS, Cowgill JB, Cui Q, Goldsmith RH, Chanda B. Structure and dynamics underlying elementary ligand binding events in human pacemaking channels. Elife. 2016 Nov 18;5PubMed PMID: 27858593; PubMed Central PMCID: PMC5115869.
  • Bao, H., Goldschen-Ohm, M., Jeggle, P., Chanda, B., Edwardson, J.M., Chapman, E.R., (2015) Exocytotic fusion pore are composed of both lipids and proteins. Nature Structural and Molecular Biology 23, 67-73  | doi:10.1038/nsmb3141.
    • Highlighted in “The mystery of the fusion pore” by Satyan Sharma and Manfred Lindau, (ibid. Pgs. 5-6).
  • Oelstrom K.M., Goldschen-Ohm, M.P., Holmgren, M., and Chanda, B. (2014) Evolutionarily conserved intracellular gate of voltage-gated sodium channels. Nature Communications DOI:10.1038/ncomms4420.
  • Capes, D.L., Goldschen-Ohm, M.P., Arcisio-Miranda, M. Bezanilla, F. and Chanda, B. (2013) Domain IV voltage-sensor is both sufficient and rate-limiting for fast inactivation in sodium channels. Journal of General Physiology 142 (2), 101-112.
    • Highlighted in “What activates inactivation?”  by Christopher Ahern, (ibid. Pgs. 97-100).
  • Goldschen-Ohm, M.P., Capes, D.L., Oelstrom, K., and Chanda, B. (2013) Multiple pore conformations driven by asynchronous movements of voltage-sensors in a eukaryotic sodium channel. Nature Communications 4:1350. doi: 10.1038/ncomms2356.

 

mpgoldschen@wisc.edu | (608) 262-3520 |

Ana Isabel Fernandez-Marino

Postdoc   2014-2016

Current Status (as of Jan 2017)- Postdoc at NIH as of 2017.

Lab Publications:

  • Fernández-Mariño, A.I., Harpole, T.J., Oelstrom, K., Delemotte, L., and Chanda, B. (2018). Gating interaction maps reveal a noncanonical electromechanical coupling mode in the Shaker K+ channel. Nature Structural & Molecular Biology 25, 320-326. DOI: 10.1038/s41594-018-0047-3. Article Link.

| | LinkedIn Page

Brian Jarecki

Postdoc   2010-2014

Current Status (as of Jan 2017) – Field Application Specialist at Cellular Dynamics International

Lab Publications:

  • Chowdhury, S., Jarecki, B.W., and Chanda, B. (2014) A molecular framework for temperature-dependent gating of ion channels. Cell 158, 1148-1158.

    • Highlighted in “Building a temperature-sensitive ion channel” by Ming-Feng Tsai and Christopher Miller, (ibid. Pgs. 977-979).
    • A video abstract was featured as PaperFlick in the online issue.
  • Jarecki, B.W., Zheng, S.L., Zhang, L., Li, X., Zhou, X., Cui, Q., Tang, W., and Chanda, B. (2013) Tethered spectroscopic probes estimate dynamic distances with sub-nanometer resolution in voltage-dependent potassium channels. Biophysical Journal 105(12) 2724-2732.

    • Featured in research highlights section in Nature Chemical Biology March 2014 (pg. 169).
    • Highlighted in “New rule(r)s for FRET”  by Frank Bosmans, (ibid. Pgs. 2619-2620).
  • Jarecki, B.W., Makino, S., Beebe, E., Fox, B., and Chanda, B. (2013) Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes. Scientific Reports 3:1040. doi: 10.1038/srep01040.
  • Capes, D., Arcisio-Miranda, M., Jarecki, B.W., French, R., and Chanda, B. (2012) Gating transitions in the selectivity filter region of a sodium channel are coupled to domain IV voltage-sensor. Proceedings of the National Academy of Sciences (USA) 109(7): 2648-53.

    • Highlighted in “Enhancement of closed-state inactivation by neutralization of S4 arginines in domain IV of a sodium channel” by Tzur Paldi (Frontiers in Pharmacology (2012) doi:10.3389/fphar.2012.00143 )

| | LinkedIn Page

Manoel Arciscio-Miranda

Postdoc   2007-2010

Current Status (as of Jan 2017)- Professor, Department of Biophysics, Federal University of Sao Paulo, Brazil.

Lab Publications:

  • Capes, D.L., Goldschen-Ohm, M.P., Arcisio-Miranda, M. Bezanilla, F. and Chanda, B. (2013) Domain IV voltage-sensor is both sufficient and rate-limiting for fast inactivation in sodium channels. Journal of General Physiology 142 (2), 101-112.

    • Highlighted in “What activates inactivation?”  by Christopher Ahern, (ibid. Pgs. 97-100).
  • Capes, D., Arcisio-Miranda, M., Jarecki, B.W., French, R., and Chanda, B. (2012) Gating transitions in the selectivity filter region of a sodium channel are coupled to domain IV voltage-sensor. Proceedings of the National Academy of Sciences (USA) 109(7): 2648-53.

    • Highlighted in “Enhancement of closed-state inactivation by neutralization of S4 arginines in domain IV of a sodium channel” by Tzur Paldi (Frontiers in Pharmacology (2012) doi:10.3389/fphar.2012.00143 )
  • Arcisio-Miranda, M., Muroi, Y., Chowdhury, S., and Chanda, B. (2010) Molecular basis of allosteric modification of voltage-dependent gating by local anesthetics. Journal of General Physiology 136(5), 541-554.
  • Muroi, Y., Arcisio-Miranda, M., Chowdhury, S., and Chanda, B. (2010) Molecular determinants of coupling between the domain III voltage-sensor and pore of a sodium channel. Nature Structural and Molecular Biology 17, 230-237.

| | Lab Website

Yukiko Muroi

Post Doc   2007-2009

Currently Instrumental Scientist – Product Performance Evaluation at L’Oréal, Japan

Lab Publications:

  • Arcisio-Miranda, M., Muroi, Y., Chowdhury, S., and Chanda, B. (2010) Molecular basis of allosteric modification of voltage-dependent gating by local anesthetics. Journal of General Physiology 136(5), 541-554.
  • Muroi, Y., Arcisio-Miranda, M., Chowdhury, S., and Chanda, B. (2010) Molecular determinants of coupling between the domain III voltage-sensor and pore of a sodium channel. Nature Structural and Molecular Biology 17, 230-237.
  • Muroi, Y., and Chanda, B. (2009) Local anesthetics disrupt energetic coupling between voltage-sensors of the sodium channel. Journal of General Physiology 133, 1-15.

| | LinkedIn Page

Former Graduate Students

Kevin Oelstrom

Graduate Student   2010-2016

Current Status (as of Jan 2017)- Scientist at Cellular Dynamics International.

Lab Publications:

  • Oelstrom, K., and Chanda, B. (2016) Congruent pattern of accessibility identifies minimal pore gate in a nonsymmetric voltage-gated sodium channel.Nature Communications DOI:10.1038/ncomms11608.
  • Oelstrom K.M., Goldschen-Ohm, M.P., Holmgren, M., and Chanda, B. (2014) Evolutionarily conserved intracellular gate of voltage-gated sodium channels. Nature Communications DOI:10.1038/ncomms4420.
  • Goldschen-Ohm, M.P., Capes, D.L., Oelstrom, K., and Chanda, B. (2013) Multiple pore conformations driven by asynchronous movements of voltage-sensors in a eukaryotic sodium channel. Nature Communications 4:1350. doi: 10.1038/ncomms2356.

| | LinkedIn Page

Sandipan Chowdhury

Graduate Student   2008- 2014

Current Status (as of Jan 2017) Jane Coffins Fellow at Eric Gouaux’s lab, Vollum Institute.

Lab Publications:

  • Chowdhury, S., Haehnal, B.M., and Chanda, B. (2014) Interfacial gating triad is crucial for electromechanical transduction in voltage-activated potassium channels. Journal of General Physiology 144:457-467.
  • Chowdhury, S., Haehnal, B.M., and Chanda, B. (2014) A self-consistent approach for determining pairwise interactions that underlie channel activation. Journal of General Physiology 144: 441-455.
  • Chowdhury, S., Jarecki, B.W., and Chanda, B. (2014) A molecular framework for temperature-dependent gating of ion channels. Cell 158, 1148-1158.
    • Highlighted in “Building a temperature-sensitive ion channel” by Ming-Feng Tsai and Christopher Miller, (ibid. Pgs. 977-979).
    • A video abstract was featured as PaperFlick in the online issue.
  • Chowdhury, S., and Chanda, B. (2013) Free energy relationships in ion channels activated by voltage and ligand. Journal of General Physiology. 141(1): 11-28.
    Highlighted in “No model in mind: A model-free approach for studying ion channel gating” by Ofer Yifrach (ibid. Pgs. 3-9).
  • Chowdhury, S., and Chanda, B. (2012) Estimating the voltage-dependent free energy change of ion channels using the median voltage of activation.Journal of General Physiology 139 (1): 3-17.
    • Highlighted in “Model-free energy for voltage-gated ion channels” by Christopher Miller (ibid. Pgs 1-2).
    • Also highlighted in “Taking a thermodynamic approach: A conversation with Baron Chanda” by Elizabeth Adler (Journal of General Physiology vol. 144, no.2, Pg.127)
  • Chowdhury, S., and Chanda, B. (2010) Deconstructing thermodynamic parameters from site-specific observables in a coupled system. Proceedings of the National Academy of Sciences (USA)  107(44), 18856-18861.
  • Arcisio-Miranda, M., Muroi, Y., Chowdhury, S., and Chanda, B. (2010) Molecular basis of allosteric modification of voltage-dependent gating by local anesthetics. Journal of General Physiology 136(5), 541-554.
  • Muroi, Y., Arcisio-Miranda, M., Chowdhury, S., and Chanda, B. (2010) Molecular determinants of coupling between the domain III voltage-sensor and pore of a sodium channel. Nature Structural and Molecular Biology 17, 230-237.

| | LinkedIn Page

Deborah Capes

Graduate Student   2008-2013

Current Status (as of Jan 2017)- Medical Writer, Covance.

Lab Publications:

  • Capes, D.L., Goldschen-Ohm, M.P., Arcisio-Miranda, M. Bezanilla, F. and Chanda, B. (2013) Domain IV voltage-sensor is both sufficient and rate-limiting for fast inactivation in sodium channels. Journal of General Physiology 142 (2), 101-112.
    • Highlighted in “What activates inactivation?”  by Christopher Ahern, (ibid. Pgs. 97-100).
  • Goldschen-Ohm, M.P., Capes, D.L., Oelstrom, K., and Chanda, B. (2013) Multiple pore conformations driven by asynchronous movements of voltage-sensors in a eukaryotic sodium channel. Nature Communications 4:1350. doi: 10.1038/ncomms2356.
  • Capes, D., Arcisio-Miranda, M., Jarecki, B.W., French, R., and Chanda, B. (2012) Gating transitions in the selectivity filter region of a sodium channel are coupled to domain IV voltage-sensor. Proceedings of the National Academy of Sciences (USA) 109(7): 2648-53.
    • Highlighted in “Enhancement of closed-state inactivation by neutralization of S4 arginines in domain IV of a sodium channel” by Tzur Paldi (Frontiers in Pharmacology (2012) doi:10.3389/fphar.2012.00143 )

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Former Undergraduates

Aindrila Saha

Summer Research Assistant (Khorana Program )   2017

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Alexander Blair

Undergraduate Researcher 2015-2018  

Current Position (as of September 2018) – Medical Student, UW-Madison

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Not Pictured: 

Megan Schramski (2016-2017)

Zachary Nuner (2014-2016)

Brady Weigel (2013-2014)

Rishi Raj Trivedi (2011)

Travers Lingle (2010-2011)

Jacob Kohlenberg (2010)

Jishnu Das (2009)

Kristina Schuldt (2007-2010)

Pjillpe Pesssoa De Santana (2008)

Eric Printz (2007)